Primer tRNAs for reverse transcription

Primer tRNAs for reverse transcription.

Both retroviruses and long terminal repeat (LTR) retrotransposons use cellular tRNAs as primers for reverse transcription during their replication cycles. In retroviruses, primer tRNA is selectively packaged into the virion, where it is placed onto the primer binding site (PBS) of the viral RNA genome and used to prime the reverse transcriptase (RT)-catalyzed synthesis of minus-strand cDNA. Stu...

Minireview Primer Trnas for Reverse Transcription

Primer tRNAs for reverse transcription.

tRNAs Promote Nuclear Import of HIV-1 Intracellular Reverse Transcription Complexes

Infection of non-dividing cells is a biological property of HIV-1 crucial for virus transmission and AIDS pathogenesis. This property depends on nuclear import of the intracellular reverse transcription and pre-integration complexes (RTCs/PICs). To identify cellular factors involved in nuclear import of HIV-1 RTCs, cytosolic extracts were fractionated by chromatography and import activity exami...

An intelligent primer design system for multiplex reverse transcription polymerase chain reaction and complementary DNA microarray

Various biological and clinical applications require the quantification of the messenger RNA (mRNA) abundance of the biological materials under investigation. Reverse transcription-polymerase chain reaction (RT-PCR) is a popular technique for mRNA quantification. However, in these applications, multiple quantifications of mRNA are required from the same sample. Multiplex RT-PCR uses multiple pr...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*